This molecular technique involves high-throughput sequencing (HTS), which facilitates the identification of multiple species using environmental DNA (eDNA) extracted from complex ecological samples (Creer et al., 2016 Taberlet et al., 2012). Metabarcoding has become an effective method for molecular-based biomonitoring programs and biosurveillance for invasive alien species (IAS Makiola et al., 2020). Our study results reaffirm the effectiveness and importance of integrating eDNA metabarcoding as part of identification protocols in biosurveillance programs. Similarly, using 16S ribosomal RNA and nuclear ribosomal internal transcribed spacer (ITS), five bacterial and three fungal genera, which contain species of regulatory concern across several Canadian jurisdictions, were recovered from all sampling locations. Two IAS ( Agrilus planipennis and Lymantria dispar) are regulated by the Canadian Food Inspection Agency (CFIA) as plant health pests, are known to occur in the study area, and were identified through eDNA in collected traps.
BINs were distributed among 57 Orders and 304 Families, with the vast majority being arthropods.
Using cytochrome c oxidase I (COI) as a molecular marker, a modified Illumina protocol effectively identified 2,535 Barcode Index Numbers (BINs). We collected insects and eDNA samples using Lindgren funnel traps that contained a saturated salt (NaCl) solution in the collection jar. Forty-eight traps were placed, following the CFIA's annual forest insect trapping survey, at four locations in southern Ontario that are high risk for forest IAS. In the present study, we optimized and validated a fast and cost-effective eDNA metabarcoding protocol for biosurveillance of IAS and characterization of insect and microorganism diversity. The early detection of IAS can allow for prompt actions by regulating authorities, thereby mitigating their impacts. The introduction of insect pests considered invasive alien species (IAS) into a non-native range poses a threat to native plant health.
Environmental DNA (eDNA) metabarcoding has revolutionized biodiversity monitoring and invasive pest biosurveillance programs.
0 kommentar(er)
